Protandim Testimonials and Science

Protandim users report positive experiences after using the product.  These testimonials are followed by research studies that explain why the product is effective. 

LifeVantage (the company):   "Protandim activates the body's natural ability to reduce age-related symptoms on the cellular level. Protandim reduces oxidative stress in humans by an average of 40 percent in 30 days. It activates Nrf2 to communicate with your DNA to deliver a biochemical wake-up call to your genes.  Certain foods, especially fruits and vegetables, contain antioxidants. But Protandim will boost your body’s production of antioxidants more effectively by activating the protein Nrf2, telling cells to turn on a network of antioxidant enzymes and protective genes."    Dozens of leading institutions are studying Protandim and how it helps people with diabetes, hypertension, skin cancer, bypass grafts, etc.  Studies show a 30% increase in Superoxide Dismutase (SOD), 54% increase in Catalase (CAT), and 300% increase in Glutathione, with regulation of over 3000 genes.  It has been featured in many popular media sources (Dr. Phil, Dr. Oz, abc primetime, PBS Healing Quest, NBC Today, etc).   See Lifevantage.com.
Nancy Levitt:  "I suffered from ADD, Joint Pain and Skin Cancer. I had real achy joints from teaching aerobics for so many years, and after just a few weeks of using Protandim, that subsided immediately. I was also using some anxiety medication for my ADD, and I noticed all my symptoms from anxiety started going away. So my doctor weened me off that medication. I feel very calm, very focused.  I no longer struggle with ADD, and joint pain is gone."  (youtube)

Andrea Boyd:  "I'm the mother of a four and a half year old who is diagnosed with high functioning Autism since he was two years old. So for the last two and a half years we've been in and out of different therapies that have been trying to get him past certain hurdles that he has been trying to get over. A couple of those hurdles are things like speech and social skills, learning how to play with others, learning how to ask for things that he wants or needs, and just very simple day to day things that normal people do to live. The therapies in the past have worked. We've done speech therapy, we've done a lot of behavioral therapy. We're still doing all those things. With the one thing that I've noticed though in the last couple of months or so he's really skyrocketed in terms of how he has progressed from the way he used to be to how he is now, more like a normal four and a half year old child would be.   The one thing that I've seen that is different is that we have been having him on the Protandim for the last eight weeks. One of the biggest things that I am really appreciative about is that I've noticed that his level of progression over the last eight weeks has been far greater than in any 8 week period that I can remember. Just putting him on that Protandim over the last couple of months has really made a huge impact on him. I've seen him want to initiate play with other kids. I've seen him come up to me and ask questions, and ask for things that he wants and he is talking in complete sentences when he has never done that before. It's just amazing to me because I have never seen a progression that fast in him. It's been absolutely amazing for him. I'm so thankful the doctor gave him the Protandim, and we're life long addicts now."   (https://www.youtube.com/watch?v=h6rOxqamQRY)

Harold Reynolds:  "Many years I've had Arthritis in my hands. They were so bad I couldn't force my fingers down. I couldn't do anything with them. They were sore all the time. In about 4 weeks taking Protandim, I can now make a fist. No pain, it's gone. I'm feeling real good. I've had a lot of other experiences with Protandim. In April I went to the doctor for my annual physical. I went to have my lab work done. So I waited to find out what the results of that was. When the doctor come in he was just shaking his head.  He said, Harold, I don't know what I'm going to do with you. He says we just got the lab work back, and according to the lab your kidneys are functioning the same as an 18 or 19 year old. For an 81 year old man that's quite a difference there. He said your liver and all your organs inside are just like a young man. I said doctor, you know what's causing that don't you? He said after reading the material about Protandim, it's got to be that. He said nothing else I know of could slow down the aging process in a person like this has done for you. So it's just amazing, and I'll tell you I'm going to stay on it for the rest of my life." (youtube)

Honolulu Aunty:  Before I started taking Protandim, I had a standard blood test done at Clinical Laboratory.  My cholesterol levels were borderline high as well as my glucose levelsBone density test was very poor and of concern enough that my doctor recommend that I start on Fosomax (which I refused and use Strontium instead).  My energy level was low during the day, many times I felt great fatigue in the afternoon even while driving and wanted to just lie down and sleep.

I felt “old” and getting older.  My ability to concentrate and continue the regular and mundane tasks of accounting, paperwork, investing, learning, shopping, organizing, etc. was beginning to feel overwhelming and I worried constantly about what I was forgetting.  I almost dreaded going to TaiChi classes because of my inability to remember how to do the steps, even though I have been going to the same class for over 2 years.  My average brain age on the brain test was about 34, occasionally 28, and sometimes 38 or higher if I was tired or it was late at night.  I woke up everyday very tired, with stiff hands and a cloudiness over my eyes, which cleared up after about 10 minutes.  I had some knee stiffness and pain, but it was not bad enough for me to take anything for it.

The experiment

Day 1.  I began taking one Protandim tablet once a day around noon from October 1, 2011.

Day 2.  I felt a bit yucky.  So did Uncle.  My nose was a bit runny for about a week, which is unusual since didn’t have a cold.

Day 3.  When I awoke, my eyes were clear.  The normal cloudiness of vision was gone.  Quite amazing.  Every morning from this point on, I wake up and can clearly see the time on the clock without any blurry vision (though I still need reading glasses).

Week 2.  Usually when I eat eggplant (in the nightshade family of veggies) my hands are extra stiff and sore in the morning.  This morning, after having eaten spicy garlic eggplant (yum yum), my hands felt normal.  No stiffness.  No soreness.

Month 2.  I feel sharper, don’t have brain fog anymore.  Even though I had missed weeks of TaiChi – my memory for the steps was far better than ever before.  My sensei Virginia commented on it – she expected me to have forgotten everything after such a long absence.

I stopped inputting into my journal after month #2.  Not because I forgot to.  It was because I was “sold” on Protandim.  It was working wonderfully for me and it is hard to report about nothingness (the state of not having issues).

Currently.  I feel more energized.  I wake up without feeling groggy or tired (quite amazing).  I very rarely need naps anymore.  My eyes have remained clear.  I continue to do many different tasks and do not feel overwhelmed.  My mental capacity seems to be sharp – I don’t feel like I am “losing” it and I am getting more done (except for my clutter).  In fact, I am taking on more busy-ness nowadays, and able to handle the mental and physical chores of additional responsibilities without the usual fatigue and stress I used to have.

My average brain age on the brain test is now 26.  Many times I score 23 when I am in the zone (more often than not), and the worst that I do nowadays is 28.

My fingernails seem stronger and are getting longer without cracking.  There is a horizontal ridgeline on my nails that would correspond to 6 months ago when I started taking Protandim.  Much to Uncle’s happiness, I also feel sexier and clothes look better on me.

The occasional pain in my knees have seemed to be gone.  The absence of pain is not as noticeable as the presence of pain.  It is quite a nice feeling.  (honoluluaunty.com)


The following are from http://gmnprotandim.com/testimonials.htm  


Toni Chester:   What i noticed twice this past week is that I have a feeling of being happy.. for me that is like nearly impossible since I suffer from chronic major depressive disorder. I caught myself twice thinking.. i feel really happy... my energy is improving but since i was so run down, that will most likely take a while for me. another improvement is that the chronic cough I have due to silent reflux went away much quicker this time. I was taking another med with the protandim, but only took one course of that.. and the cough seems to have subsided for the most part....    from http://gmnprotandim.com/testimonials.htm 

 

Al DiCicco:   "For three years my blood platelet levels have been low. The last reading in March 2014 was 109,000. I took Protandim for 5 days prior to my retest in July and levels are now 139,000 with 150,000-450,000 being normal. My Aluminum level is the lowest it has been in 3 years, reading at 5 mcg/L. I am SO excited about Protandim! This products seems to help remove Aluminum and raise blood platelets. I intend to stay on it and monitor progress with my blood work. Thank you guys so much".   http://gmnprotandim.com/testimonials.htm

Angelique Z.:   I've been on Protandim for a couple of weeks and my thoughts are definitely clearer, and I have had zero joint pain without taking my MSM. My tummy has flattened a bit and I haven't changed my diet. I've been trying to accomplish that for over ten years.   http://gmnprotandim.com/testimonials.htm

Alicia H.:   We didn't eat and take it right away this morning and by 11 we knew it. So we ate and within an hour I felt more alert. Rocky's skin is still slowly progressing. His stomach is barely a pimple and a tiny bit of raised skin. Most of the flakiness is gone all over. I also noticed Rocky is looking more youthful too, and I noticed the darkness on my lids and bags under my eyes are going away. Which is amazing because they have been always that way since childhood. I also have digestive problems. Up until recently my stomach was hurting a lot and I was worried I would have to go back to the hospital. But since taking Protandim, the pains so far have not returned. And this is just after a couple of weeks on Protandim!   http://gmnprotandim.com/testimonials.htm

 

Connie G.:   Good afternoon..well, it's taken a few days, but today I feel awesome. Lately I've been with weak muscles and no ambition.  In fact, Monday I sat on my butt in front of the TV I felt so bad. Today I'm feeling AWESOME...lots if energy. http://gmnprotandim.com/testimonials.htm

Rocky S.:   WOW, within the first five hours of taking that supplement, I noticed a big difference in my over all well being! I'm really stoked about it! THANK YOU guys SO MUCH ! We love y'all! http://gmnprotandim.com/testimonials.htm

Doree and Brian M.:   I am on day 3 on the pills and I feel much better!! Brian's energy is CRAZY too!!  I have NEVER seen him so energetic.  These are MIRACLE pills!! THANK YOU!!   http://gmnprotandim.com/testimonials.htm

 

Marie Snow:   I have been overweight for 20 years. my thyroid is no longer working and I couldn't lose weight no matter what I did. A month and a half ago I started taking Protandim and I have lost 16 lbs... my doctor was shocked that I lost so much in such a short time. And I am telling you.. I am not even trying to lose weight! I told her about Protandim and she wants to know more about it. I have also had horrible anxiety for many years and have been taking Ativan every day. I have been able to cut my dosage in half since I started Protandim and will soon be rid of the Ativan for good soon, as now I have no anxiety!   http://gmnprotandim.com/testimonials.htm

Morgan Quinn: My boyfriend’s mother is into energy healing and has made a business out of it and has helped thousands of people. She also distributes and takes a supplement called Protandim. I am in the medical field and of course me being the major sceptic I am, decided to do my research. This product seemed to have all the scientific data behind it unlike a lot of the other products I've seen that have a similar function. I gave in and decided to try it. My significant other’s mother supplied me with a bottle.

I am only 24 years old and since I was 20 I have had a horrible popping in my hips and muscle pain in that area which also causes sciatica pain (more like an annoyance).   I also have terrible TMJ which makes it hard to eat steak or anything else that is hard to chew. To get out of bed would be such a hard task. It would feel like there was an extra 20-30 pounds weighing me down and I remember walking up the stair would be such a hard task I would avoid it as much as possible.  My Sciatic Nerve pain was so bad I could have my boyfriend punch my butt so hard that it would be completely bruised. People might think that is strange but those with that have this pain can truly relate. I have been diagnosed with paraformis muscle pain and degenerative labral tissue, which is the cartilage that cushions the ball joint in the hip and is usually a sign of early arthritis, which runs in my family. I have been on many a medication that treat the symptoms. Tramadol, Meloxicam (Metacam), Ibuprofen 800, Vicoden, Prednisone, Different nerve pain meds, etc. I saw that Protandim can help with arthritis so I began taking it in hopes to treat the root cause, instead of just the symptoms....I honestly have had AMAZING results! I am still on Cymbalta, but I have stopped steroids, tramadol, barely take Ibuprofen. My hips have stopped popping. My overall pain is SO much lower. I am soon going to wean off Cymbalta (not looking forward to because of bad withdrawal symptoms). Unfortunately my jaw still pops, but it is clear as day that this supplement has done something amazing to me. I am the biggest sceptic I know, but it's hard to deny this after seeing and feeling this first hand. UPDATE: I have been taking Protandim for about 2 months now. I feel great! I am off Cymbalta (after going through horrible withdrawals from it). My hips still have a little pain but gradually I can tell they are improving. I'm so glad I found Protandim. Any one that has pain should be on this. I feel we all should be healing the cause of our ailments, not just symptoms.  Please..Please try this if you have arthritis, fibromyalgia, etc... It will change your life. (http://protandimreviews.blogspot.com/)


Jan Jan:  Jan Jan has a wonderful testimony about the product.  He gave his brother-in-law Protandim, made him drink 1 pill in the morning, and 1 pill in the evening.  This brother-in-law was diagnosed with cirrhosis of the liver, at the last stage already, and was sent home by the doctors so he can just wait for his time.  He couldn't get up anymore.  He couldn't even talk.  They had a priest administer to him the last rites, in fact.  However, 2 days after taking Protandim, he felt better, expelled a lot of "toxic waste" and after 5 days got up and asked for food.  On the 10th day, which was his birthday, he went out of the house and sang karaoke.  Amazing!  Jan Jan has photos of his progress.   (http://www.aliveandyoung.com/testimonials.html)

Wilson V.:      My sister who takes care of my Dad wrote this email to me:  "I was first introduced to this product when I had expressed to my friend my frustration over the cost of pharmaceutical drugs over a year ago.  I had just picked up 3 refills and was informed that Dad had maxed his Medicare prescription coverage of approximately $3,000 and therefore he was now in the "gap" coverage which meant higher expense for his meds.  It cost more than $100 to pick up 3 meds that day.  It  was only August so there were still 4 more months before his coverage would renew at the start of the next year.  
     He was taking 17 medications a day.  So my friend asked if I was open to trying something natural that she heard was working so well that many people were getting off of prescription medications.  I said absolutely.  What can it hurt at this point.  For the last 4 years Dad's doctor had been increasing his prescriptions due to all the side effects of each pill he was prescribing.  He started off with 6 and it went up from there.  
     If you listen to the pharmaceutical commercials they list some horrific side effects, but the doctors will always say that the positive outweighs the negative. There's only so much that our kidneys [or livers] can take but that doesn't seem to be our doctor's concern as he kept increasing Dad's medications.  I recall how tired he was and lacked energy.  I just kept believing that he was someday going to pull out of this condition since his stroke in May 2008. 
     Of course, I was open to trying a more natural approach. So Dad starting taking Protandim on August 27, 2012 faithfully, along with all 17 meds a day.  Six weeks later on October 8, 2012, my Dad went in for his routine check-up at the world-renowned Mayo Clinic.  After running many comprehensive tests, including blood work and x-rays, the doctors could not find anything wrong with him like they had before.  In fact, they said his lab results went so well that the doctor took him off of 14 of his 17 medications!  They couldn't believe it.  
     Dad continued to see his family physician while taking the usual quarterly blood work.  By January he no longer needed insulin, because of his taking Protandim.  That was, to me the best news as I hated to poke him four times a day [with a needle]. Dad was always such a trooper throughout this process. So as of August 2013, we got his Medicare coverage statements and Dad is nowhere close to the expenses he incurred last August -- thanks to the Lord who introduced this natural, God given product to us..."  
     Update October 2013: My Dad is now taking only 3 medications a day instead of 17, due to Protandim.  

My sister's email continues: "...Now our whole family is taking it.  I just turned 50 and I have enough energy to take care of Dad while taking care of myself.  I do 5 days of high-intensity Zumba classes, take a 90 minute tennis clinic (drills) in the heat of the day and play tennis again for another 2 hours during the week and then take a self-defense class twice a week.  And no, I do not have to ice my knees or crawl out of bed!  In fact, my husband and girls can attest to the fact that I can go on as little as 5-6 hours of sleep.  God is SOOO good! I just want to share this product and opportunity with everyone so they too can enjoy the life they're given because 80 is TRULY the new 20!"   (http://www.aliveandyoung.com/testimonials.html)

Glenn Kramer: Two years prior to retiring from the U.S. Postal Service in 1997, Glenn started experiencing significant pain in his knees when he had to climb a flight of steps.  Rather than depending upon typical painkillers, he went the natural route, using MSM, Chondroitin and Glucosamine (dietary supplements used for treating osteoarthritis).  As the condition continued to deteriorate, he also added OTC painkillers such as Aleve to better control the pain.  In 2009, x-rays revealed significant inflammation and arthritic conditions, although not significant enough at that time to warrant knee replacement surgery.  On February 20, 2010, he added Protandim to his regimen.  Within 2 weeks, only an occasional twinge was felt while negotiating the steps.  One week later, there was absolutely no pain!  After 4 weeks, Glenn started saving money as he discontinued using MSM, Chondroitin, and Glucosamine.  He feels blessed knowing he is now physically capable of handling upcoming mission work in Spain. (http://www.jbowers.webforcepro.net/Testimonials.html)

Dr. Kathy Yuhasz: For the past 2 years I have been limping around with left knee pain. It was painful to walk fast or straighten it out; the worst pain, however, came from bending it. I could barely sit without hanging on to tables etc. because I had to leave my leg straight to sit down.  Getting in and out of a car was insane. My knee was so tight that the slightest bend would send very painful aggravation to the sciatic nerve as well, then it would throb all day and night just because of attempting to bend it.  It would be so bad some days that I couldn’t lift my leg high enough to go upstairs.  I usually would have to hop down the stairs because bending was out of the question.   An MRI revealed only a probable problem with the ACL.  I have not been able to run with my dog since I got her because of the pain and swelling during and after.  It was even hard to walk slowly for short distances with her. IN ONLY 4 DAYS ON PROTANDIM, I ACTUALLY CAUGHT MYSELF SITTING COMFORTABLY AT THE DINNER TABLE WITH BOTH OF MY LEGS BENT PAST 90 DEGREES!!!  Since this was the only thing I did differently in the last 2 years, I would have to give the credit to Protandim for the relief I am continually experiencing. I am so grateful to have been introduced to this product for obvious personal reasons (also for the other health benefits this product offers).  BUT even more so that I can share this good news with all my patients and anyone who will take the time to learn what it can do for them.  http://www.jbowers.webforcepro.net/Testimonials.html

Craig and Linda: My husband and I have been taking Protandim now since October 23rd, 2009. After only 2 weeks he asked me if I noted any changes in myself.  Until he asked I wasn't really aware, but I noticed my prescription glasses were blurry and I could see better when I took them off.  My vision was so clear it was sometimes very stark to look about.  My husband noticed he no longer had difficulty driving at night, and we drove straight through the night to Iowa, that was amazing. Then we both noticed the snoring had completely gone away. The last thing I noticed and we have been taking Protandim now for approximately 2 months, was all the age spots I had on both my forearms have completely faded or disappeared. We are now watching and waiting for more changes as we continue on this program. We have so many friends that have lost jobs just this past year and are without insurance. Protandim is the answer to all of this.   http://www.jbowers.webforcepro.net/Testimonials.html

Randy: I began using the product personally on November 6.  I have been tracking my blood pressure, body temperature, weight, and urine ph daily (missing a few day here and there.)  I wanted to have some first hand results to share with others.  After about 6 weeks, my blood pressure has gone down from an average of over 175/100 to about 143/86.  My ph was in the 5.5-5.9 the first few days but has increased to the 6.3-6.8 range with several days logging in at over 7.0.  My weight has decreased from about 230 to 224 with no other dietary changes.  We have also been giving Protandim to my 95 year old father-in-law who lives with us and believe we are seeing improvement in his mental clarity and awareness.  My wife and our grandson are also taking the product and I can only say that we all seem to notice an overall improvement in our sense of well-being.   http://www.jbowers.webforcepro.net/Testimonials.html

John Harris: Troubled by painful, swollen, sensitive joints since 1996, John had used prescription medications, but due to cost ($300+- per month), has relied on OTC pain-killers.  The condition would come and go, occasionally lasting up to 3 months at a time.  It could be so severe that just the weight of a sheet touching his toe was excruciating.  In March of 2010, John took his first Protandim tablet on a Friday night.  By Saturday morning, he states that 80% of the pain was gone, and that by Sunday night, he was pain-free!  As of this writing (June 3, 2010), John is pleased to report that he has not had any more flare-ups, and that he sleeps much better as a side benefit!   http://www.jbowers.webforcepro.net/Testimonials.html

Jim Kemp: Enjoying good health for his first 47 years, the past 10 years have not been kind to Jim Kemp of Castle Rock, CO.  He has endured 37 different surgeries (2 from different auto accidents, and what he hopes was his last surgery in March of 2010); had cancer three times (prostate, colon, thyroid); suffered from fibromyalgia and kidney stones; had issues with the pituitary, pancreas, gall bladder, and esophagus; and dealt with NASH (non-alcoholic steatohepatitis, a fatty liver condition).  He has been subjected to numerous medications, narcotic pain killers, chemotherapy, and radiation.  2009 was particularly unkind, with 5 of those 37 surgeries and 8 hospitalizations occurring within a 9 month stretch.  

When not in the hospital, Jim’s daily routine for the past five years (2004-2009), has started at 5:00 a.m., when he would arise to take 7 medications.  Then he would go back to bed for a while, getting up later to take a hot bath for 40 minutes to relieve the muscle and joint pain and stiffness.  The balance of the day was spent coordinating the ingestion of food and additional medications—some with food, some before food, and some after food, and maneuvering around the house attached to an oxygen bottle and cannula.  At bedtime, Jim would be so revved up from the meds that he would have to take sleeping pills to get to sleep.

 Prior to starting two tablets per day of Protandim on January 10, 2010, Jim traveled to Utah to say his goodbye’s to his family, and met with his attorney to finalize his will and estate issues, as he was preparing to die.  Swallowing 25 meds each day, stooped over, jaundiced, walking with a cane, attached to an oxygen bottle 24/7, and skin turning gray, Jim didn’t hold out much hope.  However, as of March 11, 2010, Jim reports that there is:

 No cane required for walking, and no being stooped over

No oxygen required for breathing

No need for narcotic pain killers (and there were no withdrawal symptoms from discontinuing them!  An occasional Tylenol does the job now.)

No gray skin tones (and his hair color is starting to come back)

No liver spots on the back of his hands

No jaundice

No $380 per month in co-pays for medications (now $25 per month)

Only 7 pills required as of now, down from the 25 he was taking prior to January 10th (including a reduction in Lyrica from 300 mg daily down to 75 mg every 4th day)

Normal liver enzymes for the first time in 8 years

Normal blood work for the first time in 9 years

 He also reports that he now sleeps better, has more energy, and states that “I feel better than I have in years!”

 Jim’s wife, Beverly, and his two adult children rejoice in having their husband and father back again.  And Jim has returned full-time to his technical support position with Sprint Nextel.    http://www.jbowers.webforcepro.net/Testimonials.html

SOURCES OF TESTIMONIALS:  youtube.com (search protandim testimonials), honoluluaunty.com,  http://gmnprotandim.com/testimonials.htmhttp://protandimreviews.blogspot.com/,  http://www.aliveandyoung.com/testimonials.html,  http://www.jbowers.webforcepro.net/Testimonials.html



RESEARCH ARTICLE (from pubmed.gov):

Free Radic Biol Med. 2013 Oct;63:264-73. doi: 10.1016/j.freeradbiomed.2013.05.024. Epub 2013 May 27.

Nrf2 activation: a potential strategy for the prevention of acute mountain sickness.

Abstract

Reactive oxygen species (ROS) formed during acute high altitude exposure contribute to cerebral vascular leak and development of acute mountain sickness (AMS). Nuclear factor (erythroid-derived 2)-related factor 2 (Nrf2) is a transcription factor that regulates expression of greater than 90% of antioxidant genes, but prophylactic treatment with Nrf2 activators has not yet been tested as an AMS therapy. We hypothesized that prophylactic activation of the antioxidant genome with Nrf2 activators would attenuate high-altitude-induced ROS formation and cerebral vascular leak and that some drugs currently used to treat AMS symptoms have an additional trait of Nrf2 activation. Drugs commonly used to treat AMS were screened with a luciferase reporter cell system for their effectiveness to activate Nrf2, as well as being tested for their ability to decrease high altitude cerebral vascular leak in vivo. Compounds that showed favorable results for Nrf2 activation from our screen and attenuated high altitude cerebral vascular leak in vivo were further tested in brain microvascular endothelial cells (BMECs) to determine if they attenuated hypoxia-induced ROS production and monolayer permeability. Of nine drugs tested, with the exception of dexamethasone, only drugs that showed the ability to activate Nrf2 (Protandim, methazolamide, nifedipine, amlodipine, ambrisentan, and sitaxentan) decreased high-altitude-induced cerebral vascular leak in vivo. In vitro, Nrf2 activation in BMECs before 24h hypoxia exposure attenuated hypoxic-induced hydrogen peroxide production and permeability. Prophylactic Nrf2 activation is effective at reducing brain vascular leak from acute high altitude exposures. Compared to acetazolamide, methazolamide may offer better protection against AMS. Nifedipine, in addition to its known vasodilatory activities in the lung and protection against high altitude pulmonary edema, may provide protection against brain vascular leak as well.

Copyright © 2013 Elsevier Inc. All rights reserved.

PMID:  23722164  [PubMed - indexed for MEDLINE]   PMCID:  PMC4130652  Free PMC Article

[INTERPRETATION:  Free radicals (ROS) can contribute to brain blood vessel problems and sickness.  Drugs that activate Nrf2 (like Protandim) decrease the blood vessel problems.]

RESEARCH ARTICLE (from pubmed.gov):

Exerc Sport Sci Rev. 2013 Jul;41(3):162-8. doi: 10.1097/JES.0b013e3182948a1e.

The role of Nrf2 in the attenuation of cardiovascular disease.

Abstract

Oxidative stress is a component of many human diseases, including cardiovascular diseases (CVD). Exercise and various phytochemicals activate nuclear factor (erythroid-derived 2)-like 2 (Nrf2), the master regulator of antioxidant defenses, and attenuate CVD. This review highlights Nrf2 regulation by exercise and phytochemicals and the role of Nrf2 as a therapeutic target in CVD.

PMID:  23558695  [PubMed - indexed for MEDLINE]

 

[INTERPRETATION:  Various activities and substances cause Nrf2 activation.  Nrf2 regulates antioxidant defenses and decreases cardiovascular disease, so it is a good therapy for cardiovascular problems.]

RESEARCH ARTICLE (from pubmed.gov):

Free Radic Biol Med. 2013 Mar;56:102-11. doi: 10.1016/j.freeradbiomed.2012.11.016. Epub 2012 Nov 30.

Upregulation of phase II enzymes through phytochemical activation of Nrf2 protects cardiomyocytes against oxidant stress.

Abstract

Increased production of reactive oxygen species has been implicated in the pathogenesis of cardiovascular disease (CVD), and enhanced endogenous antioxidants have been proposed as a mechanism for regulating redox balance. Nuclear factor (erythroid-derived 2)-like 2 (Nrf2) is a transcriptional regulator of phase II antioxidant enzymes, and activation of Nrf2 has been suggested to be an important step in attenuating oxidative stress associated with CVD. A well-defined combination of five widely studied medicinal plants derived from botanical sources (Bacopa monniera, Silybum marianum (milk thistle), Withania somnifera (Ashwagandha), Camellia sinensis (green tea), and Curcuma longa (turmeric)) has been shown to activate Nrf2 and induce phase II enzymes through the antioxidant response element. The purpose of these experiments was to determine if treatment of cardiomyocytes with this phytochemical composition, marketed as Protandim, activates Nrf2, induces phase II detoxification enzymes, and protects cardiomyocytes from oxidant-induced apoptosis in a Nrf2-dependent manner. In cultured HL-1 cardiomyocytes, phytochemical treatment was associated with nuclear accumulation of Nrf2, significant induction of phase II enzymes, and concomitant protection against hydrogen peroxide-induced apoptosis. The protection against oxidant stress was abolished when Nrf2 was silenced by shRNA, suggesting that our phytochemical treatment worked through the Nrf2 pathway. Interestingly, phytochemical treatment was found to be a more robust activator of Nrf2 than oxidant treatment, supporting the use of the phytochemicals as a potential treatment to increase antioxidant defenses and protect heart cells against an oxidative challenge.

Copyright © 2012 Elsevier Inc. All rights reserved.

PMID:  23201694  [PubMed - indexed for MEDLINE]

[INTERPRETATION:  Free radicals (reactive oxygen species) cause heart disease, but activation of Nrf2 reduces the problems associated with the heart problems.  A combination of medicinal plants (the ingredients in Protandim) activates Nrf2, and is proven to protect against cell death.  Chemicals from plants can be a good treatment to protect heart cells.]

RESEARCH ARTICLE (from pubmed.gov):

Antioxid Redox Signal. 2013 May 10;18(14):1810-7. doi: 10.1089/ars.2012.4828. Epub 2012 Sep 28.

Antioxidants for the treatment of patients with severe angioproliferative pulmonary hypertension?

Abstract

SIGNIFICANCE:

Pathobiological mechanisms that contribute to pulmonary vasoconstriction, lung vascular remodeling, and the development of right heart failure include the generation of reactive oxygen and nitrogen species and the response of lung vascular and cardiac cells to these molecules. We review the information regarding oxidant stress balanced by antioxidant mechanisms and the role of oxidants and antioxidants in hypoxic pulmonary hypertension and their potential role in an animal model of severe pulmonary arterial hypertension (PAH).

RECENT ADVANCES:

In human lung tissue from patients with idiopathic PAH, we find reduced superoxide dismutase activity and high expression of the oxidant stress markers nitrotyrosine and 8-OH-guanosine. In the Sugen 5416/chronic hypoxia model of PAH, lung tissue expression of nitrotyrosine and hemeoxygenase 1 (HO-1) is substantial, while HO-1 expression in the failing right ventricle is decreased. This model, based on administration of the VEGF receptor blocker Sugen 5416 and chronic hypoxia (Su/Hx), reproduces many of the characteristic features of severe angioobliterative human PAH. Treatment of Su/Hx rats with protandim, which nuclear factor erythroid-2 related factor (Nrf2)-dependently upregulates the expression of genes encoding antioxidant enzymes, protects against right heart failure without affecting angioobliterative PAH.

CRITICAL ISSUES:

In human severe PAH, patient survival is determined by the function of the stressed right ventricle; investigation of oxidative and nitrosative stresses and their potential contribution to right heart failure is necessary.

FUTURE DIRECTIONS:

Antioxidant therapeutic strategies may be of benefit in the setting of human severe PAH. Whether antioxidant strategies affect lung vascular remodeling and/or prevent right heart failure remains to be examined.

PMID:  22870869  [PubMed - indexed for MEDLINE]

[INTERPRETATION:  Oxidants (reactive oxygen species) contribute to constriction of blood vessels in the lungs and heart failure.  Oxidant stress markers are found in humans with high blood pressure in their lungs.  Treatment with Protandim protects against heart failure by upregulating the body’s production of antioxidants via Nrf2.]

RESEARCH ARTICLE (from pubmed.gov):

Oxid Med Cell Longev. 2012;2012:132931. doi: 10.1155/2012/132931. Epub 2012 May 22.

Phytochemical activation of Nrf2 protects human coronary artery endothelial cells against an oxidative challenge.

Abstract

Activation of NF-E2-related factor 2 (Nrf2) is a potential therapeutic intervention against endothelial cell oxidative stress and associated vascular disease. We hypothesized that treatment with the phytochemicals in the patented dietary supplement Protandim would induce Nrf2 nuclear localization and phase II antioxidant enzyme protein in human coronary artery endothelial cells (HCAECs), protecting against an oxidant challenge in an Nrf2- dependent manner. Protandim treatment induced Nrf2 nuclear localization, and HO-1 (778% of control ± 82.25 P < 0.01), SOD1 (125.9% of control ± 6.05 P < 0.01), NQO1 (126% of control ± 6.5 P < 0.01), and GR (119.5% of control ± 7.00 P < 0.05) protein expression in HCAEC. Treatment of HCAEC with H(2)O(2) induced apoptosis in 34% of cells while pretreatment with Protandim resulted in only 6% apoptotic cells (P < 0.01). Nrf2 silencing significantly decreased the Protandim-induced increase in HO-1 protein (P < 0.01). Nrf2 silencing also significantly decreased the protection afforded by Protandim against H(2)O(2)- induced apoptosis (P < 0.01 compared to no RNA, and P < 0.05 compared to control RNA). These results show that Protandim induces Nrf2 nuclear localization and antioxidant enzyme expression, and protection of HCAEC from an oxidative challenge is Nrf2 dependent.

PMID:  22685617  [PubMed - indexed for MEDLINE]   PMCID:  PMC3364676  Free PMC Article

[INTERPRETATION:  Protandim treatment caused Nrf2 to activate, which significantly reduced cell death in coronary artery cells.  Protection against cell death by oxidants depends on Nrf2.]

RESEARCH ARTICLE (from pubmed.gov):

Am J Physiol Lung Cell Mol Physiol. 2012 Apr 1;302(7):L688-99. doi: 10.1152/ajplung.00297.2011. Epub 2012 Jan 20.

Protandim does not influence alveolar epithelial permeability or intrapulmonary oxidative stress in human subjects with alcohol use disorders.

Abstract

Alcohol use disorders (AUDs), including alcohol abuse and dependence, have been linked to the development of acute lung injury (ALI). Prior clinical investigations suggested an association between AUDs and abnormal alveolar epithelial permeability mediated through pulmonary oxidative stress that may partially explain this relationship. We sought to determine if correcting pulmonary oxidative stress in the setting of AUDs would normalize alveolar epithelial permeability in a double-blinded, randomized, placebo-controlled trial of Protandim, a nutraceutical reported to enhance antioxidant activity. We randomized 30 otherwise healthy AUD subjects to receive directly observed inpatient oral therapy with either Protandim (1,350 mg/day) or placebo. Subjects underwent bronchoalveolar lavage (BAL) and blood sampling before study drug administration and after 7 days of therapy; all AUD subjects completed the study protocol without adverse events. BAL total protein was measured at each timepoint as an indicator of alveolar epithelial permeability. In subjects with AUDs, before study drug initiation, BAL total protein values were not significantly higher than in 11 concurrently enrolled controls (P = 0.07). Over the 7-day study period, AUD subjects did not exhibit a significant change in BAL total protein, regardless of their randomization to Protandim {n = 14, -2% [intraquartile range (IQR), -56-146%]} or to placebo [n = 16, 77% (IQR -20-290%); P = 0.19]. Additionally, among those with AUDs, no significant changes in BAL oxidative stress indexes, epithelial growth factor, fibroblast growth factor, interleukin-1β, or interleukin-10 were observed regardless of drug type received. Plasma thiobarbituric acid reactive substances, a marker of lipid peroxidation, decreased significantly over time among AUD subjects randomized to placebo (P < 0.01). These results suggest that Protandim for 7 days in individuals with AUDs who are newly abstinent does not alter alveolar epithelial permeability. However, our work demonstrates the feasibility of safely conducting clinical trials that include serial bronchoscopies in a vulnerable population at risk for acute lung injury.

PMID:  22268125  [PubMed - indexed for MEDLINE]   PMCID:  PMC3330762  Free PMC Article

[INTERPRETATION: Oxidative stress (cell damage by free radicals) in the lungs has been associated with liquids entering the air sacs, causing lung injury.  7 days of Protandim did not change the amount entering the air sacs, but did show that it is safe for future experiments.]

 

RESEARCH ARTICLE (from pubmed.gov):

Mol Aspects Med. 2011 Aug;32(4-6):234-46. doi: 10.1016/j.mam.2011.10.006. Epub 2011 Oct 15.

Oxidative stress in health and disease: the therapeutic potential of Nrf2 activation.

Abstract

For the past 40 years or so, oxidative stress has been increasingly recognized as a contributing factor in aging and in various forms of pathophysiology generally associated with aging. Our view of oxidative stress has been largely "superoxide-centric", as we focused on the pathological sources of this oxygen-derived free radical and the types of molecular havoc it can wreak, as well as on the protection provided by the antioxidant enzymes, especially the superoxide dismutases, catalases, and glutathione peroxidases. In the last decade our view of oxidative stress has broadened considerably, and it is now often seen as an imbalance that has its origins in our genes, and the ways in which gene expression is regulated. At the center of this new focus is the transcription factor called nuclear factor (erythroid-derived 2)-like 2, or Nrf2. Nrf2 is referred to as the "master regulator" of the antioxidant response, modulating the expression of hundreds of genes, including not only the familiar antioxidant enzymes, but large numbers of genes that control seemingly disparate processes such as immune and inflammatory responses, tissue remodeling and fibrosis, carcinogenesis and metastasis, and even cognitive dysfunction and addictive behavior. Thus, the dysregulation of Nrf2-regulated genes provides a logical explanation for the connections, both direct and indirect, between observable oxidative stress and perhaps 200 human diseases involving these various physiological processes, each reflecting a network involving many gene products. The evolutionary self-association of these many genes under the common control of Nrf2 suggests that the immune and inflammatory systems may present the largest demand for increased antioxidant protection, apart from constitutive oxidative stress resulting from mitochondrial oxygen consumption for metabolic purposes. Gene expression microarray data on human primary vascular endothelial cells and on the SK-N-MC human neuroblastoma-derived cell line have been obtained in response to the dietary supplement Protandim, a potent composition of highly synergistic phytochemical Nrf2 activators. Pathway analysis of results shows significant modulation by Protandim of pathways involving not only antioxidant enzymes, but of those related to colon cancer, cardiovascular disease, and Alzheimer disease.

Copyright © 2011 Elsevier Ltd. All rights reserved.

PMID:  22020111  [PubMed - indexed for MEDLINE]   Free full text

 

[INTERPRETATION:  Scientists have known for 40 years that oxidative stress causes aging and the diseases associated with aging, that free radicals destroy cells, and that the body’s own antioxidants protect.  In the last 10 years scientists have learned that Nrf2 regulates the antioxidants and causes genes to control immunity, inflammation, tissue repair, cancer growth, cognition, and addiction.  Poor Nrf2 activation has direct and indirect connections with about 200 human diseases.  Immunity and inflammation need the most antioxidant protection offered by Nrf2.  Protandim helps the body produce antioxidants and helps with colon cancer, heart problems, and Alzheimer’s.]

RESEARCH ARTICLE (from pubmed.gov):

Enzyme Res. 2011;2011:409295. doi: 10.4061/2011/409295. Epub 2011 Mar 23.

The role of manganese superoxide dismutase in skin cancer.

Abstract

Recent studies have shown that antioxidant enzyme expression and activity are drastically reduced in most human skin diseases, leading to propagation of oxidative stress and continuous disease progression. However, antioxidants, an endogenous defense system against reactive oxygen species (ROS), can be induced by exogenous sources, resulting in protective effects against associated oxidative injury. Many studies have shown that the induction of antioxidants is an effective strategy to combat various disease states. In one approach, a SOD mimetic was applied topically to mouse skin in the two-stage skin carcinogenesis model. This method effectively reduced oxidative injury and proliferation without interfering with apoptosis. In another approach, Protandim, a combination of 5 well-studied medicinal plants, was given via dietary administration and significantly decreased tumor incidence and multiplicity by 33% and 57%, respectively. These studies suggest that alterations in antioxidant response may be a novel approach to chemoprevention. This paper focuses on how regulation of antioxidant expression and activity can be modulated in skin disease and the potential clinical implications of antioxidant-based therapies.

PMID:  21603266  [PubMed]   PMCID:  PMC3092576  Free PMC Article

[INTERPRETATION:  Scientists have recently proven that most people who have skin diseases have low body-produced antioxidants, so oxidative stress (cell destruction by oxidants) and disease progression continue.  However, foods and other orally-taken substances can cause more antioxidants to be produced, resulting in protection and fighting of the diseases.  Protandim decreased the incidence of skin tumors by 33% and reduced the spread of tumors by 57%.  Protandim may be a new approach to cancer prevention.]

RESEARCH ARTICLE (from pubmed.gov):

Free Radic Biol Med. 2011 Mar 15;50(6):700-9. doi: 10.1016/j.freeradbiomed.2010.12.008. Epub 2010 Dec 15.

Protandim attenuates intimal hyperplasia in human saphenous veins cultured ex vivo via a catalase-dependent pathway.

Abstract

Human saphenous veins (HSVs) are widely used for bypass grafts despite their relatively low long-term patency. To evaluate the role of reactive oxygen species (ROS) signaling in intima hyperplasia (IH), an early stage pathology of vein-graft disease, and to explore the potential therapeutic effects of up-regulating endogenous antioxidant enzymes, we studied segments of HSV cultured ex vivo in an established ex vivo model of HSV IH. Results showed that HSV cultured ex vivo exhibit an ~3-fold increase in proliferation and ~3.6-fold increase in intimal area relative to freshly isolated HSV. Treatment of HSV during culture with Protandim, a nutritional supplement known to activate Nrf2 and increase the expression of antioxidant enzymes in several in vitro and in vivo models, blocks IH and reduces cellular proliferation to that of freshly isolated HSV. Protandim treatment increased the activity of SOD, HO-1, and catalase 3-, 7-, and 12-fold, respectively, and decreased the levels of superoxide (O(2)(•-)) and the lipid peroxidation product 4-HNE. Blocking catalase activity by cotreating with 3-amino-1,2,4-triazole abrogated the protective effect of Protandim on IH and proliferation. In conclusion, these results suggest that ROS-sensitive signaling mediates the observed IH in cultured HSV and that up-regulation of endogenous antioxidant enzymes can have a protective effect.

Copyright © 2010 Elsevier Inc. All rights reserved.

PMID:  21167278  [PubMed - indexed for MEDLINE]

[INTERPRETATION:  Protandim activates Nrf2, which increases antioxidants, which block early-stage disease in vein grafts.  This up-regulation of antioxidant enzymes  has protective properties.]

RESEARCH ARTICLE (from pubmed.gov):

J Diet Suppl. 2010 Jun 1;7(2):159-178.

The Dietary Supplement Protandim Decreases Plasma Osteopontin and Improves Markers of Oxidative Stress in Muscular Dystrophy Mdx Mice.

Abstract

Therapeutic options for Duchenne muscular dystrophy (DMD), the most common and lethal neuromuscular disorder in children, remain elusive. Oxidative damage is implicated as a pertinent factor involved in its pathogenesis. Protandim((R)) is an over-the-counter supplement with the ability to induce antioxidant enzymes. In this study we investigated whether Protandim((R)) provided benefit using surrogate markers and functional measures in the dystrophin-deficient (mdx)mouse model of DMD. Male 3-week-old mdx mice were randomized into two treatment groups: control (receiving standard rodent chow) and Protandim((R))-supplemented standard rodent chow. The diets were continued for 6-week and 6-month studies. The endpoints included the oxidative stress marker thiobarbituric acid-reactive substances (TBARS), plasma osteopontin (OPN), plasma paraoxonase (PON1) activity, H&E histology, gadolinium-enhanced magnetic resonance imaging (MRI) of leg muscle and motor functional measurements. The Protandim((R)) chow diet in mdx mice for 6 months was safe and well tolerated. After 6 months of Protandim((R)), a 48% average decrease in plasma TBARS was seen; 0.92 nmol/mg protein in controls versus 0.48 nmol/mg protein in the Protandim((R)) group (p = .006). At 6 months, plasma OPN was decreased by 57% (p = .001) in the Protandim((R))-treated mice. Protandim((R)) increased the plasma antioxidant enzyme PON1 activity by 35% (p = .018). After 6 months, the mdx mice with Protandim((R)) showed 38% less MRI signal abnormality (p = .07) than mice on control diet. In this 6-month mdx mouse study, Protandim((R)) did not significantly alter motor function nor histological criteria.

PMID:  20740052  [PubMed]   PMCID: PMC2926985  Free PMC Article

[INTERPRETATION:  In the past there has been no good treatment for Muscular Dystrophy.  It is caused by oxidative damage.  The good news is that Protandim is beneficial because it induces antioxidants that reduce the oxidative damage.  Protandim is proven to significantly decrease oxidative stress (as measured by several tests: 48% and 57% decreases), and at the same time increases antioxidants by 35%, and causes 38% less abnormality.]

RESEARCH ARTICLE (from pubmed.gov):

PLoS One. 2010 Jul 30;5(7):e11902. doi: 10.1371/journal.pone.0011902.

The chemopreventive effects of Protandim: modulation of p53 mitochondrial translocation and apoptosis during skin carcinogenesis.

Abstract

Protandim, a well defined dietary combination of 5 well-established medicinal plants, is known to induce endogenous antioxidant enzymes, such as manganese superoxide dismutase (MnSOD). Our previous studies have shown through the induction of various antioxidant enzymes, products of oxidative damage can be decreased. In addition, we have shown that tumor multiplicity and incidence can be decreased through the dietary administration of Protandim in the two-stage skin carcinogenesis mouse model. It has been demonstrated that cell proliferation is accommodated by cell death during DMBA/TPA treatment in the two-stage skin carcinogenesis model. Therefore, we investigated the effects of the Protandim diet on apoptosis; and proposed a novel mechanism of chemoprevention utilized by the Protandim dietary combination. Interestingly, Protandim suppressed DMBA/TPA induced cutaneous apoptosis. Recently, more attention has been focused on transcription-independent mechanisms of the tumor suppressor, p53, that mediate apoptosis. It is known that cytoplasmic p53 rapidly translocates to the mitochondria in response to pro-apoptotic stress. Our results showed that Protandim suppressed the mitochondrial translocation of p53 and mitochondrial outer membrane proteins such as Bax. We examined the levels of p53 and MnSOD expression/activity in murine skin JB6 promotion sensitive (P+) and promotion-resistant (P-) epidermal cells. Interestingly, p53 was induced only in P+ cells, not P- cells; whereas MnSOD is highly expressed in P- cells when compared to P+ cells. In addition, wild-type p53 was transfected into JB6 P- cells. We found that the introduction of wild-type p53 promoted transformation in JB6 P- cells. Our results suggest that suppression of p53 and induction of MnSOD may play an important role in the tumor suppressive activity of Protandim.

PMID:  20689586  [PubMed - indexed for MEDLINE]   PMCID: PMC2912769  Free PMC Article

[INTERPRETATION:  Protandim activates body-produced antioxidants, which reduces oxidative stress, which reduces many problems.  Protandim decreases tumor growth and spreading and stops skin cell death.  Protandim suppresses tumors because it suppresses the mechanisms of cell death and increases protective antioxidant enzymes.]

RESEARCH ARTICLE (from pubmed.gov):

Circulation. 2009 Nov 17;120(20):1951-60. doi: 10.1161/CIRCULATIONAHA.109.883843. Epub 2009 Nov 2.

Chronic pulmonary artery pressure elevation is insufficient to explain right heart failure.

Abstract

BACKGROUND:

The most important determinant of longevity in pulmonary arterial hypertension is right ventricular (RV) function, but in contrast to experimental work elucidating the pathobiology of left ventricular failure, there is a paucity of data on the cellular and molecular mechanisms of RV failure.

METHODS AND RESULTS:

A mechanical animal model of chronic progressive RV pressure overload (pulmonary artery banding, not associated with structural alterations of the lung circulation) was compared with an established model of angioproliferative pulmonary hypertension associated with fatal RV failure. Isolated RV pressure overload induced RV hypertrophy without failure, whereas in the context of angioproliferative pulmonary hypertension, RV failure developed that was associated with myocardial apoptosis, fibrosis, a decreased RV capillary density, and a decreased vascular endothelial growth factor mRNA and protein expression despite increased nuclear stabilization of hypoxia-induced factor-1alpha. Induction of myocardial nuclear factor E2-related factor 2 and heme-oxygenase 1 with a dietary supplement (Protandim) prevented fibrosis and capillary loss and preserved RV function despite continuing pressure overload.

CONCLUSIONS:

These data brought into question the commonly held concept that RV failure associated with pulmonary hypertension is due strictly to the increased RV afterload.

PMID:  19884466  [PubMed - indexed for MEDLINE]   Free full text

[INTERPRETATION:  Old beliefs about the cause of heart failure are changing.  This study shows that Protandim activates Nrf2, which prevents stiffening of heart muscles, keeps blood flow up, and preserves heart function.  It is assumed then that the cause of heart failure could be oxidative stress, because that is what Nrf2 causes to be reduced.]

RESEARCH ARTICLE (from pubmed.gov):

PLoS One. 2009;4(4):e5284. doi: 10.1371/journal.pone.0005284. Epub 2009 Apr 22.

Protandim, a fundamentally new antioxidant approach in chemoprevention using mouse two-stage skin carcinogenesis as a model.

Abstract

Oxidative stress is an important contributor to cancer development. Consistent with that, antioxidant enzymes have been demonstrated to suppress tumorigenesis when being elevated both in vitro and in vivo, making induction of these enzymes a more potent approach for cancer prevention. Protandim, a well-defined combination of widely studied medicinal plants, has been shown to induce superoxide dismutase (SOD) and catalase activities and reduce superoxide generation and lipid peroxidation in healthy human subjects. To investigate whether Protandim can suppress tumor formation by a dietary approach, a two-stage mouse skin carcinogenesis study was performed. At the end of the study, the mice on a Protandim-containing basal diet had similar body weight compared with those on the basal diet, which indicated no overt toxicity by Protandim. After three weeks on the diets, there was a significant increase in the expression levels of SOD and catalase, in addition to the increases in SOD activities. Importantly, at the end of the carcinogenesis study, both skin tumor incidence and multiplicity were reduced in the mice on the Protandim diet by 33% and 57% respectively, compared with those on basal diet. Biochemical and histological studies revealed that the Protandim diet suppressed tumor promoter-induced oxidative stress (evidenced by reduction of protein carbonyl levels), cell proliferation (evidenced by reduction of skin hyperplasia and suppression of PKC/JNK/Jun pathway), and inflammation (evidenced by reduction of ICAM-1/VCAM-1 expression, NF-kappaB binding activity, and nuclear p65/p50 levels). Overall, induction of antioxidant enzymes by Protandim may serve as a practical and potent approach for cancer prevention.

PMID:  19384424  [PubMed - indexed for MEDLINE]   PMCID: PMC2668769  Free PMC Article

[INTERPRETATION:  Cancer starts with oxidative stress (cell death by oxidants).  Body-produced antioxidants suppress the start of tumors.  Protandim increases the body’s antioxidants, so it can prevent tumors.  Test results show that tumor incidence was reduced by 33% and tumor multiplicity was reduced by 57%.  The reason is because Protandim suppressed 3 things: the oxidative stress, the spreading of cells, and inflammation.  Protandim is an easy and potent approach to prevent cancer.]

RESEARCH ARTICLE (from pubmed.gov):

Free Radic Biol Med. 2009 Feb 1;46(3):430-40. doi: 10.1016/j.freeradbiomed.2008.10.050. Epub 2008 Nov 17.

Synergistic induction of heme oxygenase-1 by the components of the antioxidant supplement Protandim.

Abstract

Protandim is an antioxidant supplement that consists of five ingredients, namely, ashwagandha, bacopa extract, green tea extract, silymarin, and curcumin, each with known therapeutic properties. Protandim was formulated with the objective of combining multiple phytochemicals at low nontoxic doses to gain synergy among them. A recent clinical study demonstrated the in vivo antioxidant effects of Protandim (S.K. Nelson et al., 2006, Free Radic. Biol. Med. 40, 341-347). The objective of the present study was to determine if the components of Protandim induce heme oxygenase-1 (HO-1) in a synergistic manner in cultured MIN6 cells, a mouse beta-cell line, and in SK-N-MC cells, a human neuroblastoma cell line. When the components of Protandim were tested alone at low doses, curcumin showed minimal induction, whereas the others were unable to induce the HO-1 promoter, assayed by transient transfection. All components together, however, produced a strongly synergistic induction of around three- to ninefold in a dose-dependent manner, greatly exceeding the sum of the parts. Similar findings were obtained for the expression of HO-1 at the mRNA and protein levels. Protandim-mediated HO-1 induction involved the presence of ARE sites in the HO-1 promoter and nuclear translocalization of the transcription factor Nrf2, which binds to ARE sites. The involvement of multiple signaling pathways, including PI3-kinase/Akt, p38MAPK, and PKCdelta, in HO-1 induction seems to be the probable mechanism of synergy between the components of Protandim. There were significant increases in the levels of total glutathione in Protandim-treated cells. These findings suggest that the use of a combination of phytochemicals may be an efficient method for the induction of antioxidant enzymes.

PMID:  19056485  [PubMed - indexed for MEDLINE]

[INTERPRETATION:  Protandim has antioxidant effects.  The ingredients combined together greatly exceeded the sum of their parts in antioxidant activity, indicating that a combination is the best method.  Also, Protandim significantly increased the levels of glutathione (a powerful antioxidant) in cells.]

RESEARCH ARTICLE (from pubmed.gov):

Free Radic Biol Med. 2006 Jan 15;40(2):341-7.

The induction of human superoxide dismutase and catalase in vivo: a fundamentally new approach to antioxidant therapy.

Abstract

A composition consisting of extracts of five widely studied medicinal plants (Protandim) was administered to healthy human subjects ranging in age from 20 to 78 years. Individual ingredients were selected on the basis of published findings of induction of superoxide dismutase (SOD) and/or catalase in rodents in vivo, combined with evidence of decreasing lipid peroxidation. Each ingredient was present at a dosage sufficiently low to avoid any accompanying unwanted pharmacological effects. Blood was analyzed before supplementation and after 30 and 120 days of supplementation (675 mg/day). Erythrocytes were assayed for SOD and catalase, and plasma was assayed for lipid peroxidation products as thiobarbituric acid-reacting substances (TBARS), as well as uric acid, C-reactive protein, and cholesterol (total, LDL, and HDL). Before supplementation, TBARS showed a strong age-dependent increase. After 30 days of supplementation, TBARS declined by an average of 40% (p = 0.0001) and the age-dependent increase was eliminated. By 120 days, erythrocyte SOD increased by 30% (p < 0.01) and catalase by 54% (p < 0.002). We conclude that modest induction of the catalytic antioxidants SOD and catalase may be a much more effective approach than supplementation with antioxidants (such as vitamins C and E) that can, at best, stoichiometrically scavenge a very small fraction of total oxidant production.

PMID:  16413416  [PubMed - indexed for MEDLINE]

[INTERPRETATION:  Protandim’s ingredients were selected because they induce body-produced antioxidants, but were combined at a low enough dose to prevent unwanted side-effects.  Oxidative stress (cell destruction by free radicals) was reduced by an average of 40%.  Signs of aging were reduced to the youngest person’s level (age 20).  The antioxidant SOD increased by 30% and the antioxidant catalase increased by 54%.  The best way to reduce cell destruction is to activate the body’s own antioxidants – not by taking vitamin C, E, etc.]


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